image   Products for Metabolic Profiling

Isotopic Ratio Outlier Analysis (IROA®) is a simple and direct metabolic labeling protocol provided in kit form, for the in-vitro incorporation of a specific and unique signature or tag into biochemical metabolites for global metabolic identification and relative quantitation by mass spectrometry (MS).   IROA® can be applied to many life science applications (toxicology, diagnostics, drug development, bioprocess, etc.) as the technique is amenable to most cell populations. 

For situations where it is not practical or possible to label experimental samples in cell culture systems such as biopsies, field-grown plants or large-scale fermentation, please refer to the IROA® Phenotypic Experimental Protocol section.

To determine the flux of a specific precusor through metabolic pathways, the IROA® Fluxomic Quantitation Kit may be used. 

The IROA® Quantitation Series 300 Kit provides reagents and tools for the successful labeling, identification, and quantitiation of biochemical compounds in mammalian cell populations  when combined with sample preparation and MS.  When working with fungal and bacterial cell populations, please refer to the IROA® Quantitation series 100 and 200 kits, respectively. 

 

Our Biochemical Quantitation Kits include:

  • Labeling media and components

  • Internal Labeled Standard generated for every metabolite measured!

  • ClusterFinder™ software characterizes peaks (artifacts, control, experimental),  removes artifacts, calculates carbon number and MF, quantitates control/experimental ratio and normalizes dataset

  • Access to IROA Portal’s data interpretation and statistical analysis for a total metabolomics solution

Cell Types or organisms labeled with IROA media

CHO, C. elegans, Dendritic cells, E. coli (common strains and genetically modified), Fibroblasts, HaCat, HC-04, HepG2, HL60, Jurkat, Lactobacillus, Leukemia-like cell lines, Liver cancer cell lines, Macrophages, Mel-3, Myxococcus xanthus, OVAR-8, Plasmodium, Yeast (common strains and genetically modified).