MS Authentic Standards

IROA Technologies now provides 5 sets of Metabolite Library of Standards (MLS) - all supplied with MLSDiscovery software tool which builds authentic libraries by supporting the extraction, manipulation and storage of the data when using the MLS plates.

Organic Acid Metabolite Library of Standards (OAMLS)

96 high-quality standards, convenient 96 well format, 5 µg per compound

Fatty Acid Metabolite Library of Standards (FAMLS)

96 high-quality standards, convenient 96 well format, 5 µg per compound

Bile Acid - Carnitine - Sterol Metabolite Library of Standards (BACSMSL)

96 high-quality standards, convenient 96 well format, 5 µg per compound

Mass Spectrometry Metabolite Library of Standards (MSMLS)

600 high-quality standards, convenient 96 well format, 5 µg per compound

Large Scale Metabolite Library of Standards (LSMLS)

504 high-quality standards, convenient 96 well format, 1 mg per compound
  

 

Enabling Clinical Metabolomics
How reproducible is your data?
 
Most metabolomics data can realistically only be compared with same-day-run samples, enough for small research projects but not suitable for large clinically-relevant metabolomics projects.  Clinically relevant metabolomics requires accurate identification, completely reproducible quantitation, and performance criteria for instrumentation.  How can this be achieved?
 
IROA TruQuant IQQ uses a set of complex internal standards that deal with for ionization inefficiencies/matrix effects to provide suppression-corrected data, complete cross-platform quantitative reproducibility, with validated compound identification, and continuous instrument QA/QC. 
  
Cross platform normalization of measurements utilizing the same or different analytical methodologies can be overcome using TruQuant Long-Term sets of complex metabolite C13-labeled internal standards which provide the means for data standardization and also enhancements in data quality through the removal of background/noise, quality assurance, compound identification and quantitation.

The IROA TruQuant IQQ workflow provides a software solution together with carefully formulated Long-Term Reference Standard (IROA-LTRS) containing over 500 compounds, based on 95% and 5% C13.  Why 95%/5%? The compounds in the LTRS are computationally easy to find, provide completely verified compound identify, and serve as a daily measure of instrumentation performance (complete QA/QC).  The matching IROA Internal Standard (IROA-IS) has the same chemical profile as the IROA-LTRS but acts as an internal standard to corresponding natural abundance isotopomers in any sample.  Therefore, the IROA-LTRS provides validated compound identity (I), and quality assurance (Q), the IROA-IS provides accurate calibrated Quantitation (Q) for hundreds of compounds in a single metabolomic-style measurement.  For this reason, we have named this workflow IROA TruQuant IQQ. 

 

 
 

 

TruQuant Corrects for Ion Suppression

Researchers at MD Anderson Cancer Center have tested and demonstrated that the IROA Truquant IQQ (ID-Quant-QC) Workflow can be used to correct for ion suppression over a range of metabolite concetrations.  Furthermore they showed that almost all compounds exhibit some level of suppression and compounds are suppressed to different extents between HILIC and RPC-MS.